pacap38 peptide (Millipore)
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90
Structured Review
Millipore
pacap38 peptide
![(A) Western blotting analysis of AR55Q in HEK293T cells treated with PACAP and peptides 2, 4, and 7. Quantification is shown at the bottom. n = 3 to 5 independent experiments; *P < 0.05. (B) XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] assay in MN-1 cells expressing AR65Q treated with peptides 2, 4, and 7 and 100 nM PACAP for 48 hours. n = 3 independent experiments. Veh, vehicle. (C) Real-time polymerase chain reaction (PCR) analysis of the transcript levels of Pac1, Vpac1, and Vpac2 receptors in the spinal cord and skeletal muscle of 180-day-old control (CTR; wild type) and AR113Q mice and normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1) and Pgk1 in spinal cord and to Hprt1 and β-glucuronidase (Gusβ) in skeletal muscle. n = 6 mice for each group. (D) cAMP level analysis in the spinal cord of control mice treated with PACAP27, <t>PACAP38,</t> peptide 2, and peptide 7. n = 3 mice for each group. Graphs, means ± SEM; one-way ANOVA was used.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9029/pmc11349029/pmc11349029__nihms-2017166-f0006.jpg)
Pacap38 Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pacap38+peptide/pacap38+peptide/pmc11349029-752-34-36
Average 90 stars, based on 1 article reviews
Pacap38 Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pacap38+peptide/pacap38+peptide/pmc11349029-752-34-36
Average 90 stars, based on 1 article reviews
pacap38 peptide - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy"
Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy
Journal: Science translational medicine
doi: 10.1126/scitranslmed.aaf9526
Figure Legend Snippet: (A) Western blotting analysis of AR55Q in HEK293T cells treated with PACAP and peptides 2, 4, and 7. Quantification is shown at the bottom. n = 3 to 5 independent experiments; *P < 0.05. (B) XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] assay in MN-1 cells expressing AR65Q treated with peptides 2, 4, and 7 and 100 nM PACAP for 48 hours. n = 3 independent experiments. Veh, vehicle. (C) Real-time polymerase chain reaction (PCR) analysis of the transcript levels of Pac1, Vpac1, and Vpac2 receptors in the spinal cord and skeletal muscle of 180-day-old control (CTR; wild type) and AR113Q mice and normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1) and Pgk1 in spinal cord and to Hprt1 and β-glucuronidase (Gusβ) in skeletal muscle. n = 6 mice for each group. (D) cAMP level analysis in the spinal cord of control mice treated with PACAP27, PACAP38, peptide 2, and peptide 7. n = 3 mice for each group. Graphs, means ± SEM; one-way ANOVA was used.
Techniques Used: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Control
Related Articles
Mouse Assay:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Western Blot:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Expressing:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Real-time Polymerase Chain Reaction:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Control:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. In Vivo:Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy Article Snippet: Mice were treated with vehicle [intranasal solution containing 7.5 mg of NaCl, 1.7 mg of citric acid monohydrate, 3 mg of disodium phosphate dihydrate, and 0.2 mg of benzalkonium chloride solution (50%) per milliliter; Article Title: Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Article Snippet: Dulbecco Telethon Institute, Centre for Integrative Biology, University of Trento, 38123 Trento, Italy.. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.. Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. |